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Measuring Binding Constants of His-Tagged Proteins Using Affinity. The E coli contaminants display high affinity to divalent nickel or cobalt ions mainly. Purified Water Excipients 25 USP and NF Excipients Listed by Functional Category. 20 ml Bio-rad column with cap and ring stand to hold Ni-NTA agarose Short microfuge.

Competent cells including recombinant protein purification

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